期刊
CELL REPORTS
卷 17, 期 6, 页码 1607-1620出版社
CELL PRESS
DOI: 10.1016/j.celrep.2016.10.011
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资金
- NIH [R01CA166447, P30CA016086]
- University Cancer Research Fund
- V Foundation for Cancer Research
- Wide Open Foundation
- Corn-Hammond Fund for Pediatric Oncology
- Initiative for Maximizing Student Diversity (IMSD) [5R25GM055336]
- [T32GM007092]
Chromatin regulation is critical for differentiation and disease. However, features linking the chromatin environment of stem cells with disease remain largely unknown. Weexplored chromatin accessibility in embryonic and multipotent stem cells and unexpectedly identified widespread chromatin accessibility at repetitive elements. Integrating genomic and biochemical approaches, we demonstrate that these sites of increased accessibility are associated with well-positioned nucleosomes marked by distinct histone modifications. Differentiation is accompanied by chromatin remodeling at repetitive elements associated with altered expression of genes in relevant developmental pathways. Remarkably, we found that the chromatin environment of Ewing sarcoma, a mesenchymally derived tumor, is shared with primary mesenchymal stem cells (MSCs). Accessibility at repetitive elements in MSCs offers a permissive environment that is exploited by the critical oncogene responsible for this cancer. Our data demonstrate that stem cells harbor a unique chromatin landscape characterized by accessibility at repetitive elements, a feature associated with differentiation and oncogenesis.
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