期刊
CELL REPORTS
卷 16, 期 2, 页码 295-303出版社
CELL PRESS
DOI: 10.1016/j.celrep.2016.06.003
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资金
- NIH [U24NS51870, R01GM086458]
- JSPS KAKENHI [26890025]
- Uehara Memorial Foundation
- Ruth K. Broad Biomedical Research Foundation
- Japan Society for the Promotion of Science
- Grants-in-Aid for Scientific Research [26890025] Funding Source: KAKEN
Here, we describe a targeted reverse genetic screen for thermal nociception genes in Drosophila larvae. Using laser capture microdissection and microarray analyses of nociceptive and non-nociceptive neurons, we identified 275 nociceptor-enriched genes. We then tested the function of the enriched genes with nociceptor-specific RNAi and thermal nociception assays. Tissue-specific RNAi targeted against 14 genes caused insensitive thermal nociception while targeting of 22 genes caused hypersensitive thermal nociception. Previously uncategorized genes were named for heat resistance (i.e., boiler-man, fire dancer, oven mitt, trivet, thawb, and bunker gear) or heat sensitivity (firelighter, black match, eucalyptus, primacord, jet fuel, detonator, gasoline, smoke alarm, and jetboil). Insensitive nociception phenotypes were often associated with severely reduced branching of nociceptor neurites and hyper-branched dendrites were seen in two of the hypersensitive cases. Many genes that we identified are conserved in mammals.
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