期刊
CELL REPORTS
卷 17, 期 10, 页码 2715-2723出版社
CELL PRESS
DOI: 10.1016/j.celrep.2016.11.028
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资金
- University of Cambridge
- Hutchison Whampoa Limited
- Cancer Research UK
- ERC consolidator grant [646876]
- EMBO young investigator award
- European Research Council (ERC) [646876] Funding Source: European Research Council (ERC)
- Cancer Research UK [20411] Funding Source: researchfish
FOXA1 is a pioneer factor that binds to enhancer regions that are enriched in H3K4mono-and dimethylation (H3K4me1 and H3K4me2). We performed a FOXA1 rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME) screen in ERapositive MCF-7 breast cancer cells and found histone-lysine N-methyltransferase (MLL3) as the top FOXA1-interacting protein. MLL3 is typically thought to induce H3K4me3 at promoter regions, but recent findings suggest it may contribute to H3K4me1 deposition. We performed MLL3 chromatin immunoprecipitation sequencing (ChIP-seq) in breast cancer cells, and MLL3 was shown to occupy regions marked by FOXA1 occupancy and H3K4me1 and H3K4me2. MLL3 binding was dependent on FOXA1, indicating that FOXA1 recruits MLL3 to chromatin. MLL3 silencing decreased H3K4me1 at enhancer elements but had no appreciable impact on H3K4me3 at enhancer elements. We propose a mechanism whereby the pioneer factor FOXA1 recruits the chromatin modifier MLL3 to facilitate the deposition of H3K4me1 histone marks, subsequently demarcating active enhancer elements.
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