期刊
CELL REPORTS
卷 16, 期 11, 页码 2953-2966出版社
CELL PRESS
DOI: 10.1016/j.celrep.2016.08.023
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资金
- Dutch Cancer Society [KUN2009-4402]
- Radboudumc RIMLS PhD grant
- Netherlands Organisation for Scientific Research [951.03.002, 822.02.017]
- NWO [Veni 863.13.024, Vidi 864.11.006, Vici 918.14.655]
- NWO Spinoza grant
- ERC Adv grant PATHFINDER [269019]
Dendritic cells (DCs) play a key role in orchestrating adaptive immune responses. In human blood, three distinct subsets exist: plasmacytoid DCs (pDCs) and BDCA3+ and CD1c+ myeloid DCs. In addition, a DC-like CD16+ monocyte has been reported. Although RNA-expression profiles have been previously compared, protein expression data may provide a different picture. Here, we exploited label-free quantitative mass spectrometry to compare and identify differences in primary human DC subset proteins. Moreover, we integrated these proteomicdata with existing mRNA data to derive robust cell-specific expression signatures withmore than 400 differentially expressed proteins between subsets, forming a solid basis for investigation of subset-specific functions. We illustrated this by extracting subset identification markers and by demonstrating that pDCs lack caspase-1 and only express low levels of other inflammasome-related proteins. In accordance, pDCs were incapable of interleukin (IL)-1 beta secretion in response to ATP.
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