期刊
CELL REPORTS
卷 17, 期 10, 页码 2672-2686出版社
CELL PRESS
DOI: 10.1016/j.celrep.2016.11.029
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资金
- EMBO Young Investigator Programme
- Cancer Research UK
- Francis Crick Institute from Cancer Research UK [FC10212]
- UK Medical Research Council
- Wellcome Trust
- European Research Council [ERC-ADG-666400]
- Cancer Research UK [11582] Funding Source: researchfish
- The Francis Crick Institute [10368, 10216, 10212] Funding Source: researchfish
Cytokinesis, the final step of cell division, begins with the formation of a cleavage furrow. How the mitotic spindle specifies the furrow at the equator in animal cells remains unknown. Current models propose that the concentration of the RhoGEF ECT2 at the spindle midzone and the equatorial plasma membrane directs furrow formation. Using chemical genetic and optogenetic tools, we demonstrate that the association of ECT2 with the plasma membrane during anaphase is required and sufficient for cytokinesis. Local membrane targeting of ECT2 leads to unilateral furrowing, highlighting the importance of local ECT2 activity. ECT2 mutations that prevent centralspindlin binding compromise concentration of ECT2 at the midzone and equatorial membrane but sustain cytokinesis. While the association of ECT2 with the plasma membrane is essential for cytokinesis, our data suggest that ECT2 recruitment to the spindle midzone is insufficient to account for equatorial furrowing and may act redundantly with yet-uncharacterized signals.
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