期刊
CELL REPORTS
卷 15, 期 5, 页码 1100-1110出版社
CELL PRESS
DOI: 10.1016/j.celrep.2016.03.087
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资金
- NIH [R01 GM073850]
- NIH/University of Colorado, Boulder Molecular Biophysics Training Program [T32 GM06103]
- National Science Foundation [CHE 1266416, PHY 1125844]
- National Institute for Standards and Technology
Riboswitches are mRNA elements regulating gene expression in response to direct binding of a metabolite. While these RNAs are increasingly well understood with respect to interactions between receptor domains and their cognate effector molecules, little is known about the specific mechanistic relationship between metabolite binding and gene regulation by the downstream regulatory domain. Using a combination of cell-based, biochemical, and biophysical techniques, we reveal the specific RNA architectural features enabling a cobalamin-dependent hairpin loop docking interaction between receptor and regulatory domains. Furthermore, these data demonstrate that docking kinetics dictate a regulatory response involving the coupling of translation initiation to general mechanisms that control mRNA abundance. These results yield a comprehensive picture of how RNA structure in the riboswitch regulatory domain enables kinetically constrained liganddependent regulation of gene expression.
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