期刊
CELL REPORTS
卷 14, 期 4, 页码 723-736出版社
CELL PRESS
DOI: 10.1016/j.celrep.2015.12.067
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资金
- Swedish Strategic Foundation (SSF)
- Swedish Research Council [VR/2012-2014/521-2011-3377, VR/2011-2013/14251]
- Swedish Academy of Sciences (KVA, Soderberg Foundation)
- Karolinska Institutet-DPA
- EU Seventh-Framework Programs (EviMalar Network of Excellence)
- OIST Graduate University
- Erik and Edith Fernstrom foundation
Plasmodium falciparum virulence is associated with sequestration of infected erythrocytes. Microvascular binding mediated by PfEMP1 in complex with non-immune immunoglobulin M (IgM) is common among parasites that cause both severe childhood malaria and pregnancy-associated malaria. Here, we present cryo-molecular electron tomography structures of human IgM, PfEMP1 and their complex. Three-dimensional reconstructions of IgM reveal that it has a dome-like core, randomly oriented Fab(2)s units, and the overall shape of a turtle. PfEMP1 is a C- shaped molecule with a flexible N terminus followed by an arc-shaped backbone and a bulky C terminus that interacts with IgM. Our data demonstrate that the PfEMP1 binding pockets on IgM overlap with those of C1q, and the bulkiness of PfEMP1 limits the capacity of IgM to interact with PfEMP1. We suggest that P. falciparum exploits IgM to cluster PfEMP1 into an organized matrix to augment its affinity to host cell receptors.
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