期刊
CELL REPORTS
卷 15, 期 2, 页码 247-255出版社
CELL PRESS
DOI: 10.1016/j.celrep.2016.03.025
关键词
-
类别
资金
- Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA Award [GM088342, GM105431, U01HG007912]
- Chinese National Natural Science Foundation [61332014, 61272121]
- China Scholarship Council fellowship
- Alfred P. Sloan Foundation fellowship
- [F32GM109630]
- [P20GM103436]
- [RO1GM08809]
- [R01AR066741]
Alternative splicing (AS) plays a critical role in cell fate transitions, development, and disease. Recent studies have shown that AS also influences pluripotency and somatic cell reprogramming. We profiled transcriptome-wide AS changes that occur during reprogramming of fibroblasts to pluripotency. This analysis revealed distinct phases of AS, including a splicing program that is unique to transgene-independent induced pluripotent stem cells (iPSCs). Changes in the expression of AS factors Zcchc24, Esrp1, Mbnl1/2, and Rbm47 were demonstrated to contribute to phase-specific AS. RNA-binding motif enrichment analysis near alternatively spliced exons provided further insight into the combinatorial regulation of AS during reprogramming by different RNA-binding proteins. Ectopic expression of Esrp1 enhanced reprogramming, in part by modulating the AS of the epithelial specific transcription factor Grhl1. These data represent a comprehensive temporal analysis of the dynamic regulation of AS during the acquisition of pluripotency.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据