Site-selective radiolabeling using mushroom tyrosinase and the strain-promoted oxidation-controlled 1,2-quinone cycloaddition

We report the in vitro characterization and in vivo evaluation of a novel Zr-89-labeled radioimmunoconjugate synthesized using a site-selective bioconjugation strategy based on the oxidation of tyrosinase residues exposed by the deglycosylation of the IgG and the subsequent strain-promoted oxidation-controlled 1,2-quinone cycloaddition between these amino acids and trans-cyclooctene-bearing cargoes. More specifically, we site-selectively modified a variant of the A33 antigen-targeting antibody huA33 with the chelator desferrioxamine (DFO), thereby producing an immunoconjugate (DFO-(SPOCQ)huA33) with equivalent antigen binding affinity to its parent immunoglobulin but attenuated affinity for the Fc gamma RI receptor. This construct was subsequently radiolabeled with [Zr-89]Zr4+ to create a radioimmunoconjugate - [Zr-89]Zr-DFO-(SPOCQ)huA33 - in high yield and specific activity that exhibited excellent in vivo behavior in two murine models of human colorectal carcinoma.

Automated summary

We characterized and evaluated a novel Zr-89-labeled radioimmunoconjugate using a site-selective bioconjugation strategy. The radioimmunoconjugate was synthesized by modifying an A33 antigen-targeting antibody with the chelator desferrioxamine (DFO) and subsequently radiolabeling with [Zr-89]Zr4+. It exhibited excellent in vivo behavior in murine models of human colorectal carcinoma.