期刊
PERTANIKA JOURNAL OF TROPICAL AGRICULTURAL SCIENCE
卷 46, 期 2, 页码 373-384出版社
UNIV PUTRA MALAYSIA PRESS
DOI: 10.47836/pjtas.46.2.01
关键词
Aeromonas hydrophila; LD50; pangasius catfish; Pangasius nasutus; pathogenicity
This study investigates the pathogenicity of A. hydrophila in P. nasutus, a high-value native fish. The LD50-240hr value of A. hydrophila was found to be 0.8 x 104 CFU/ml, and the mortality rate increased with higher bacterial concentrations. Histopathological analysis revealed congestion, ascites, enlarged gall bladder, and swollen spleen in infected fish.
Pangasius catfish, Pangasius nasutus, is a promising candidate for aquaculture due to its high market value. However, the presence of pathogenic bacteria in Aeromonas hydrophila is a major concern in P. nasutus farming in this country. This study determines the pathogenicity of A. hydrophila in P. nasutus. A total of 80 P. nasutus juveniles were intraperitoneally injected with 0, 103, 105, and 107 CFU mL-1 ofA. hydrophila and monitored until 240 hr. The infected moribund fish's kidneys, livers, and spleens were collected for histopathological analysis. The LD50-240hr value was found at 0.8 x 104 CFU/ml of A. hydrophila. The percentage of mortality in 0, 103, 105, and 107 CFU/ml infected groups were found to be at 0, 40, 60, and 90%, respectively. The infected fish showed congestion at the base of the fin, ascites, enlarged gall bladder, and swollen spleen. It is the earliest report on A. hydrophila's pathogenicity in high-value native fish, P. nasutus.
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