☆ 4.6 Review

Advances in deoxyribonucleic acid extraction techniques and point-of-care molecular diagnosis of foodborne pathogens

ANALYST (2023)

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Summary: Rapid and low-cost molecular analysis is crucial for early and specific diagnostics. Automated molecular diagnostic devices, such as sample-to-answer microfluidic devices, promise a complete molecular diagnosis system. The extraction process in such devices is laborious but important for sensitive and specific detection. Thus, researching automated or semiautomated extraction methodologies is beneficial for improving extraction procedures.

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Paper-based nucleic acid sample preparation for point-of-care diagnostics

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Synthesis of low-viscosity hydrophobic magnetic deep eutectic solvent: Selective extraction of DNA

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Nucleic acid sample preparation from whole blood in a paper microfluidic device using isotachophoresis

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Ultrafast bacterial cell lysis using a handheld corona treater and loop-mediated isothermal amplification for rapid detection of foodborne pathogens

Mengzhe Li et al.

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Hydrophobic magnetic deep eutectic solvent: Synthesis, properties, and application in DNA separation

Xuelian Wang et al.

Summary: In this study, four hydrophobic magnetic deep eutectic solvents (HMDESs) were designed and prepared for DNA extraction, with the conformations of HMDESs simulated and their H-bonding interactions investigated using density functional theory (DFT) calculations. The physical and thermal properties of HMDESs were characterized, and DNA extracted by HMDESs could be quickly collected by an external magnet. Three auxiliary extraction methods were introduced, and factors impacting the DNA extraction process were systematically investigated through single-factor experimental analysis. The proposed extraction method successfully extracted DNA from complex matrices and E. coli cell lysate, and the binding sites between HMDESs and DNA were studied.

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Guanidinium Hydrophobic Magnetic Ionic Liquid-Based Dispersive Droplet Extraction for the Selective Extraction of DNA

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Guanhua Chen et al.

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Vancomycin-dendrimer based multivalent magnetic separation nanoplatforms combined with multiplex quantitative PCR assay for detecting pathogenic bacteria in human blood

Xiaoyan Feng et al.

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Magnetic Bead Chain-Based Continuous-Flow DNA Extraction for Microfluidic PCR Detection of Salmonella

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Summary: A new magnetic extraction method combined with microfluidic PCR was developed for detecting Salmonella, with an extraction efficiency of over 90% and a detection limit of 10(2) CFU/mL, promising rapid, sensitive, and simultaneous detection of multiple foodborne pathogens.

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Assay for Listeria monocytogenes cells in whole blood using isotachophoresis and recombinase polymerase amplification

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