CysDB: a human cysteine database based on experimental quantitative chemoproteomics

Cysteine chemoproteomics provides proteome-wide portraits of the ligandability or potential druggabilityfor thousands of cysteine residues. Consequently, these studies are facilitating resources for closing the druggability gap, namely, achieving pharmacological manipulation of -96% of the human proteome that re-mains untargeted by U.S. Food and Drug Administration (FDA) approved small molecules. Recent interactive datasets have enabled users to interface more readily with cysteine chemoproteomics datasets. However, these resources remain limited to single studies and therefore do not provide a mechanism to perform cross-study analyses. Here we report CysDB as a curated community-wide repository of human cysteine chemoproteomics data derived from nine high-coverage studies. CysDB is publicly available at https:// backuslab.shinyapps.io/cysdb/ and features measures of identification for 62,888 cysteines (24% of the cys-teinome), as well as annotations of functionality, druggability, disease relevance, genetic variation, and struc-tural features. Most importantly, we have designed CysDB to incorporate new datasets to further support the continued growth of the druggable cysteinome.

Automated summary

CysDB is a curated community-wide repository of human cysteine chemoproteomics data derived from nine high-coverage studies. It provides measures of identification for 62,888 cysteines (24% of the cysteinome), as well as annotations of functionality, druggability, disease relevance, genetic variation, and structural features. CysDB is designed to incorporate new datasets and support the continued growth of the druggable cysteinome.