In this study, a fluorescent probe with good cell penetration and high selectivity and sensitivity for detecting hydrazine was designed. The probe showed high selectivity and sensitivity to hydrazine in water samples, food, and in vitro and in vivo tests. The probe achieved ratiometric detection of hydrazine by ring opening using naphthalimide as the fluorescence chromophore and pyrone as the recognition site. The added ester improved the lipid solubility of the probe and enabled better penetration of the cell membrane for fluorescent imaging in cells.
Hydrazine (N2H4) is an important reagent in the field of fine chemical engineering. However, its accumulation in the environment and food chain could pose a great threat to food safety and human health. Therefore, designing a fluorescent probe with good cell penetration and high selectivity and sensitivity to detect N2H4 in actual samples and in vivo is a meaningful project. Herein, due to the nucleophilicity of hydrazine, we utilized naphthalimide as the fluorescence chromophore and pyrone as the recognition site to achieve the ratiometric detection of hydrazine by ring opening. In addition, we introduced the ester to improve the lipid solubility of the probe, which allowed the probe to better penetrate the cell membrane to realize the fluorescent imaging of probes in cells. Meanwhile, to our delight, the probe showed high selectivity and sensitivity to N2H4 in the test system, so we further applied the probe in water samples and food, in vitro and in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据