期刊
JOURNAL OF PLANT PATHOLOGY
卷 -, 期 -, 页码 -出版社
SPRINGER
DOI: 10.1007/s42161-023-01425-x
关键词
Turnip mosaic virus; Passion fruit; Potyvirus; Phylogenetic analysis
Contigs similar to turnip mosaic virus (TuMV) were identified in a diseased passion fruit plant in Fujian Province, China, through deep sequencing of extracted small RNAs. The complete genome of TuMV, named TuMV-FJ, was determined based on these contigs and Sanger sequencing. The TuMV-FJ genome is 9,833 nucleotides in length and contains an open reading frame encoding a polypeptide with 3164 amino acid residues. TuMV-FJ belongs to a world-B phylogenetic group and shares over 94% sequence identity with its closest relative, BJ-B01. An RT-LAMP assay was developed for rapid detection of TuMV-FJ. This study reports the first complete genome sequence of TuMV infecting P. edulis and provides a sensitive detection method using RT-LAMP.
Contigs with sequence identities to turnip mosaic virus (TuMV) were detected by deep sequencing of the small RNAs extracted from a passion fruit (Passiflora edulis) plant showing virus-like symptoms in Fujian Province, China. The complete genome of the TuMV, designated TuMV-FJ here, was determined based on the sequences of these contigs and Sanger sequencing of RT-PCR and 5 & PRIME;/3' RACE amplicons. The TuMV-FJ genome consists of 9,833 nucleotides excluding the poly(A) tail. It contains an open reading frame encoding a polypeptide with 3164 amino acid residues. The polypeptide was predicted to be cleaved into ten functional proteins by viral proteases. Phylogenetic analysis indicated that TuMV-FJ is a member of a world-B phylogenetic group of TuMV. TuMV-FJ shared more than 94% nucleotide and amino acid sequence identities with its closest relative, namely BJ-B01, a TuMV isolate from Brassica oleracea. A RT-LAMP assay was developed to facilitate the molecular detection of TuMV-FJ. As far as we know, this is the first report of the complete genome sequence of TuMV infecting P. edulis. In addition, the RT-LAMP assay provides a rapid, robust and highly sensitive approach for the detection of TuMV in passion fruit.
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