Clinicopathological and Electrophoretic Pattern of Serum Protein Alterations in Acute Pneumonic Sheep

The goals of this study were to determine serum protein fractions, acute phase proteins and inflammatory cytokine concentrations, as well as histological alterations, phagocytic activity, and index in sheep with pneumonia. An overall 50 adult sheep were assessed and categorized into control healthy (Group 1, n=20), and sheep with acute pneumonia (Group 2, n = 30). Pneumonia is medically diagnosed by physical examinations, ultrasonography, as well as histopathological investigation. Serum protein fraction concentrations were measured by protein electrophoresis, while inflammatory biomarkers were measured by immunoassays. globulins, immunoglobulin G, immunoglobulin M, haptoglobin, serum amyloid A, and inflammatory cytokines (Interleukin-1 & beta;, interleukin-6, and tumor necrosis factor-& alpha;) were significantly increased while albumin and Interleukin-10 were decreased in acute pneumonic group than healthy group. Phagocytic activity and index were significantly decreased in diseased sheep compared to healthy control ones. Histopathological examination of lung tissues revealed thickening of interalveolar septa, congestion, alveolar hemorrhages, hemosiderophages, and lymphocytes and macrophages infiltration. In conclusion, acute pneumonia is associated with a severe pro-inflammatory condition that enhances the release of inflammatory cytokines, resulting in organ dysfunction and mortality in sheep. With strong sensitivity and specificity, serum haptoglobin and serum amyloid A concentrations act as diagnostic biomarkers for pneumonia in sheep.

Automated summary

The study aimed to investigate the serum protein fractions, acute phase proteins, and inflammatory cytokine concentrations in sheep with pneumonia. A total of 50 adult sheep were divided into control healthy and acute pneumonia groups. The results showed increased levels of globulins, immunoglobulins, haptoglobin, serum amyloid A, and inflammatory cytokines in the pneumonia group, while albumin and Interleukin-10 were decreased. Phagocytic activity and index were also significantly lower in diseased sheep. Histopathological examination revealed various lung tissue abnormalities. In conclusion, acute pneumonia in sheep is associated with a severe pro-inflammatory condition and organ dysfunction, and serum haptoglobin and serum amyloid A concentrations can serve as diagnostic biomarkers.