期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 13, 页码 -出版社
MDPI
DOI: 10.3390/ijms241311007
关键词
amyloid bodies; intrinsically disordered protein; liquid-liquid phase separation; membrane-less organelle; nuclear stress bodies; nucleolar biomolecular condensates
In this study, the formation and functioning of membrane-less organelles, A-bodies and nSBs, were investigated. It was found that these organelles have different morphology and function, as well as differences in protein composition and propensity for liquid-liquid phase separation (LLPS). Weak interactions play a crucial role in the formation and functioning of these important organelles.
Radical changes in the idea of the organization of intracellular space that occurred in the early 2010s made it possible to consider the formation and functioning of so-called membrane-less organelles (MLOs) based on a single physical principle: the liquid-liquid phase separation (LLPS) of biopolymers. Weak non-specific inter- and intramolecular interactions of disordered polymers, primarily intrinsically disordered proteins, and RNA, play a central role in the initiation and regulation of these processes. On the other hand, in some cases, the maturation of MLOs can be accompanied by a liquid-gel phase transition, where other types of interactions can play a significant role in the reorganization of their structure. In this work, we conducted a bioinformatics analysis of the propensity of the proteomes of two membrane-less organelles, formed in response to stress in the same compartment, for spontaneous phase separation and examined their intrinsic disorder predispositions. These MLOs, amyloid bodies (A-bodies) formed in the response to acidosis and heat shock and nuclear stress bodies (nSBs), are characterized by a partially overlapping composition, but show different functional activities and morphologies. We show that the proteomes of these biocondensates are differently enriched in proteins, and many have high potential for spontaneous LLPS that correlates with the different morphology and function of these organelles. The results of these analyses allowed us to evaluate the role of weak interactions in the formation and functioning of these important organelles.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据