期刊
ADVANCED HEALTHCARE MATERIALS
卷 5, 期 11, 页码 1299-1309出版社
WILEY
DOI: 10.1002/adhm.201500824
关键词
bone regeneration; copper nanocomposites; graphene oxides; Hif-1; vascularization
资金
- National Basic Research Program of China (973 Program) [2012CB933600]
- National Science Fund for Distinguished Young Scholars of China [81225006]
- National Natural Science Foundation of China [81430012, 81170939]
- Natural Science Foundation of Shanghai [15ZR1425300]
- Shanghai Science and Technology Innovation Action Plan [15JC1490600]
- Ph.D. innovation fund from Shanghai Jiao Tong University School of Medicine [BXJ201331]
Graphene has been studied for its in vitro osteoinductive capacity. However, the in vivo bone repair effects of graphene-based scaffolds remain unknown. The aqueous soluble graphene oxide-copper nanocomposites (GO-Cu) are fabricated, which are used to coat porous calcium phosphate (CaP) scaffolds for vascularized bone regeneration. The GO-Cu nanocomposites, containing crystallized CuO/Cu2O nanoparticles of approximate to 30 nm diameters, distribute uniformly on the surfaces of the porous scaffolds and maintain a long-term release of Cu ions. In vitro, the GO-Cu coating enhances the adhesion and osteogenic differentiation of rat bone marrow stem cells (BMSCs). It is also found that by activating the Erk1/2 signaling pathway, the GO-Cu nanocomposites upregulate the expression of Hif-1 in BMSCs, resulting in the secretion of VEGF and BMP-2 proteins. When transplanted into rat with critical-sized calvarial defects, the GO-Cu-coated calcium phosphate cement (CPC) scaffolds (CPC/GO-Cu) significantly promote angiogenesis and osteogenesis. Moreover, it is observed via histological sections that the GO-Cu nanocomposites are phagocytosed by multinucleated giant cells. The results suggest that GO-Cu nanocomposite coatings can be utilized as an attractive strategy for vascularized bone regeneration.
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