期刊
ADVANCED HEALTHCARE MATERIALS
卷 5, 期 16, 页码 2113-2121出版社
WILEY
DOI: 10.1002/adhm.201600300
关键词
aortic valve; hydrodynamic performance; replacement; textile reinforcement; tissue-engineering
资金
- People Programme (Marie Curie Actions) of the European Union under REA [317512]
- Integrated Interdisciplinary Institute of Technology for Medicine (I3TM) of RWTH Aachen University - Seed-Fund [SF_14-4-08]
- START-Program of the Medical Faculty of RWTH Aachen University - Fond [691348]
The mechanical properties of tissue-engineered heart valves still need to be improved to enable their implantation in the systemic circulation. The aim of this study is to develop a tissue-engineered valve for the aortic position - the BioTexValve - by exploiting a bio-inspired composite textile scaffold to confer native-like mechanical strength and anisotropy to the leaflets. This is achieved by multifilament fibers arranged similarly to the collagen bundles in the native aortic leaflet, fixed by a thin electrospun layer directly deposited on the pattern. The textile-based leaflets are positioned into a 3D mould where the components to form a fibrin gel containing human vascular smooth muscle cells are introduced. Upon fibrin polymerization, a complete valve is obtained. After 21 d of maturation by static and dynamic stimulation in a custom-made bioreactor, the valve shows excellent functionality under aortic pressure and flow conditions, as demonstrated by hydrodynamic tests performed according to ISO standards in a mock circulation system. The leaflets possess remarkable burst strength (1086 mmHg) while remaining pliable; pronounced extracellular matrix production is revealed by immunohistochemistry and biochemical assay. This study demonstrates the potential of bio-inspired textile-reinforcement for the fabrication of functional tissue-engineered heart valves for the aortic position.
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