期刊
ADVANCED HEALTHCARE MATERIALS
卷 5, 期 17, 页码 2214-2226出版社
WILEY-BLACKWELL
DOI: 10.1002/adhm.201600212
关键词
hyperbranched polyglycerol; mitochondria targeting; NIR ZCIS; ZnS QDs; photostability; tumor theranostics
资金
- National Natural Science Foundation of China [51373117, 51573128, 51303126]
- Key Project of Tianjin Natural Science Foundation [13JCZDJC33200, 15JCQNJC03100]
- National High Technology Program of China [2012AA022603]
- Doctoral Base Foundation of the Educational Ministry of China [20120032110027]
- [SFB 1112]
Considering the critical role of mitochondria in the life and death of cells, non-invasive long-term tracking of mitochondria has attracted considerable interest. However, a high-performance mitochondria-specific labeling probe with high photostability is still lacking. Herein a highly photostable hyperbranched polyglycerol (hPG)-based near-infrared (NIR) quantum dots (QDs) nanoplatform is reported for mitochondria-specific cell imaging. Comprising NIR Zn-Cu-In-S/ZnS QDs as extremely photostable fluorescent labels and alkyl chain (C-12)/triphenylphosphonium (TPP)-functionalized hPG derivatives as protective shell, the tailored QDs@hPG-C-12/TPP nanoprobe with a hydrodynamic diameter of about 65 nm exhibits NIR fluorescence, excellent biocompatibility, good stability, and mitochondria-targeted ability. Cell uptake experiments demonstrate that QDs@hPG-C-12/TPP displays a significantly enhanced uptake in HeLa cells compared to nontargeted QDs@hPG-C-12. Further co-localization study indicates that the probe selectively targets mitochondria. Importantly, compared with commercial deep-red mitochondria dyes, QDs@hPG-C-12/TPP possesses superior photostability under continuous laser irradiation, indicating great potential for long-term mitochondria labeling and tracking. Moreover, drug-loaded QDs@hPG-C-12/TPP display an enhanced tumor cell killing efficacy compared to nontargeted drugs. This work could open the door to the construction of organelle-targeted multifunctional nanoplatforms for precise diagnosis and high-efficient tumor therapy.
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