期刊
ADVANCED HEALTHCARE MATERIALS
卷 5, 期 9, 页码 1025-1039出版社
WILEY
DOI: 10.1002/adhm.201500888
关键词
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资金
- Army Research Office Award through the Frederick Seitz Materials Research Laboratory at the University of Illinois at Urbana-Champaign [W911NF-13-0489]
- Wyss Institute in the School of Engineering and Applied Sciences at Harvard University
3D hydrogel scaffolds are widely used in cellular microcultures and tissue engineering. Using direct ink writing, microperiodic poly(2-hydroxyethylmethacrylate) (pHEMA) scaffolds are created that are then printed, cured, and modified by absorbing 30 kDa protein poly-L-lysine (PLL) to render them biocompliant in model NIH/3T3 fibroblast and MC3T3-E1 preosteoblast cell cultures. Spatial light interference microscopy (SLIM) live cell imaging studies are carried out to quantify cellular motilities for each cell type, substrate, and surface treatment of interest. 3D scaffold mechanics is investigated using atomic force microscopy (AFM), while their absorption kinetics are determined by confocal fluorescence microscopy (CFM) for a series of hydrated hydrogel films prepared from prepolymers with different homopoly mer-to-monomer (M-r) ratios. The observations reveal that the inks with higher M-r values yield relatively more open-mesh gels due to a lower degree of entanglement. The biocompatibility of printed hydrogel scaffolds can be controlled by both PLL content and hydrogel mesh properties.
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