A method to map single-cell lineages in the mouse brain by CRISPR-based barcoding

We developed CREST (CRISPR editing-based lineage-specific tracing) to enable high-throughput mapping of single-cell lineages in any Cre lineage of interest in mice. In addition, we delineated a comprehensive lineage landscape of the developing mouse ventral midbrain, revealing novel differentiation trajectories and molecular programs underlying neural specification.

Automated summary

We developed a technique called CREST (CRISPR editing-based lineage-specific tracing) for high-throughput mapping of single-cell lineages in mice. In addition, we revealed a comprehensive lineage landscape of the developing mouse ventral midbrain, uncovering novel differentiation trajectories and molecular programs involved in neural specification.