Identification and Alternative Splicing Profile of the Raffinose synthase Gene in Grass Species

Raffinose synthase (Rafs) is an important enzyme in the synthesis pathway of raffinose from sucrose and galactinol in higher plants and is involved in the regulation of seed development and plant responses to abiotic stresses. In this study, we analyzed the Rafs families and profiled their alternative splicing patterns at the genome-wide scale from 10 grass species representing crops and grasses. A total of 73 Rafs genes were identified from grass species such as rice, maize, foxtail millet, and switchgrass. These Rafs genes were assigned to six groups based the phylogenetic analysis. We compared the gene structures, protein domains, and expression patterns of Rafs genes, and also unraveled the alternative transcripts of them. In addition, different conserved sequences were observed at these putative splice sites among grass species. The subcellular localization of PvRafs5 suggested that the Rafs gene was expressed in the cytoplasm or cell membrane. Our findings provide comprehensive knowledge of the Rafs families in terms of genes and proteins, which will facilitate further functional characterization in grass species in response to abiotic stress.

Automated summary

In this study, the authors analyzed the Rafs families and their alternative splicing patterns in 10 grass species. They identified 73 Rafs genes and classified them into six groups. The gene structures, protein domains, expression patterns, and alternative transcripts of the Rafs genes were compared. The subcellular localization of a Rafs gene was also determined. This study provides comprehensive knowledge of the Rafs families in grass species and their response to abiotic stress.