期刊
ACS SYNTHETIC BIOLOGY
卷 5, 期 7, 页码 733-740出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.6b00068
关键词
optogenetics; bacterial two-component system; UV-violet light; light-regulated gene expression
资金
- National Science Foundation [EFRI-1137266]
- Office of Naval Research [MURI N000141310074]
- Welch Foundation [C-1856]
- Directorate For Engineering
- Emerging Frontiers & Multidisciplinary Activities [1137266] Funding Source: National Science Foundation
We have previously engineered green/red and red/far red photoreversible E. coli phytochrome and cyanobacteriochrome (CBCR) two-component systems (TCSs) and utilized them to program tailor-made gene expression signals for gene circuit characterization. Here, we transport the UV-violet/green photoreversible CBCR TCS UirS-UirR from Synechocystis PCC6803 to E. coli. We demonstrate that the promoter of the small RNA csiR1, previously shown to be activated by inorganic carbon stress, is a UirS-UirR output. Additionally, in contrast to a recently proposed sequestration model, we show that the sensor histidine kinase UirS phosphorylates the response regulator UirR to activate P-csiR1 transcription in response to UV-violet light. Finally, we measure changes in UirS-UirR output minutes after a change in light input and exploit these rapid dynamics to program a challenging gene expression signal with high predictability. UirS-UirR is the first engineered transcriptional regulatory tool activated exclusively by UV-violet light, and the most blue shifted photoreversible transcriptional regulatory tool.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据