期刊
ACS SYNTHETIC BIOLOGY
卷 5, 期 4, 页码 321-333出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.5b00176
关键词
titration; oscillations; bistability; monomeric regulator; delays; synthetic biology; RNA
资金
- National Science Foundation [CMMI-1266402]
- Directorate For Engineering [1266402] Funding Source: National Science Foundation
- Div Of Civil, Mechanical, & Manufact Inn [1266402] Funding Source: National Science Foundation
Molecular titration is emerging as an important biochemical interaction mechanism within synthetic devices built with nucleic acids and the CRISPR/Cas system. We show that molecular titration in the context of feedback circuits is a suitable mechanism to enhance the emergence of oscillations and bistable behaviors. We consider biomolecular modules that can be inhibited or activated by input monomeric regulators; the regulators compete with constitutive titrating species to determine the activity of their target. By tuning the titration rate and the concentration of titrating species, it is possible to modulate the delay and convergence speed of the transient response, and the steepness and dead zone of the stationary response of the modules. These phenomena favor the occurrence of oscillations when modules are interconnected to create a negative feedback loop; bistability is favored in a positive feedback interconnection. Numerical simulations are supported by mathematical analysis showing that the capacity of the closed loop systems to exhibit oscillations or bistability is structural.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据