Programmable Modulation of Membrane Permeability of Proteinosome upon Multiple Stimuli Responses

Membrane permeability is a necessary and overarching attribute for all the hollow microcompartments toward the application as nanoreactors or artificial cells. Differing from the widely reported various stimuli models, in this study we describe a way to generate a multistimuli proteinosome capable of being triggered in sequence of temperature, redox species, and pH, thus, showing a continuous modulation on the membrane permeability. Studies showed that the molecular weight cutoff of the constructed proteinosome membrane could be continuously turned up from 78 to 102 kDa and to 142 kDa, and then turned down to 35.2 kDa upon different stimuli. As a proof of concept, such continuous modulated behavior allows a well-controlled programmed release upon the encapsulation of a FITC-labeled dextran into proteinosomes. It is anticipated that such designed proteinosomes equipped with programmed modulation of membrane permeability are promising candidates for the further development of artificial model design, such as cellular communication or metabolism in which stuff exchange is required to support in situ procedures.