Anthelmintic activity of European fern extracts against Haemonchus contortus

Most drugs used in the treatment of helminthiasis in humans and animals have lost their efficacy due to the development of drug-resistance in helminths. Moreover, since anthelmintics, like many pharmaceuticals, are now recognized as hazardous contaminants of the environment, returning to medicinal plants and their products represents an environmentally friendly way to treat helminthiasis. The goal of the present study was to test the anthelminthic activity of methanol extracts of eight selected European ferns from the genera Dryopteris, Athyrium and Blechnum against the nematode Haemonchus contortus, a widespread parasite of small ruminants. Eggs and adults of H. contortus drug-susceptible strain ISE and drug-resistant strain WR were isolated from experimentally infected sheep. The efficacy of fern extracts was assayed using egg hatch test and adults viability test based on ATP-level measurement. Among the ferns tested, only Dryopteris aemula extract (0.2 mg/mL) inhibited eggs hatching by 25% in comparison to control. Athyrium distentifolium, Dryopteris aemula and Dryopteris cambrensis were effective against H. contortus adults. In concentration 0.1 mg/mL, A. distentifolium, D. aemula, D. cambrensis significantly decreased the viability of females from ISE and WR strains to 36.2%, 51.9%, 32.9% and to 35.3%, 27.0%, 23.3%, respectively in comparison to untreated controls. None of the extracts exhibited toxicity in precise cut slices from ovine liver. Polyphenol's analysis identified quercetin, kaempferol, luteolin, 3-hydroxybenzoic acid, caffeic acid, coumaric acid and protocatechuic acid as the major components of these anthelmintically active ferns.

Automated summary

Most drugs used in the treatment of helminthiasis have become ineffective due to drug resistance in helminths. Using medicinal plants and their products provides an environmentally friendly approach to treat helminthiasis. In this study, methanol extracts from eight selected European ferns were tested for their anthelminthic activity against the nematode Haemonchus contortus. Among the ferns tested, Dryopteris aemula extract inhibited egg hatching and Athyrium distentifolium, Dryopteris aemula, and Dryopteris cambrensis were effective against H. contortus adults. Major components of these anthelmintically active ferns were identified as quercetin, kaempferol, luteolin, 3-hydroxybenzoic acid, caffeic acid, coumaric acid, and protocatechuic acid.