期刊
CURRENT RESEARCH IN MICROBIAL SCIENCES
卷 4, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.crmicr.2022.100179
关键词
Plasmodium falciparum; IP 3 receptor; Malaria; Signaling; MDR transporter
类别
In this study, a potential candidate for IP3 receptor in Plasmodium falciparum, a protein associated with multidrug resistance, was identified through a combination of biochemical and bioinformatic analyses.
Intracellular Ca2+ mobilization induced by second messenger IP3 controls many cellular events in most of the eukaryotic groups. Despite the increasing evidence of IP3-induced Ca2+ in apicomplexan parasites like Plasmodium, responsible for malaria infection, no protein with potential function as an IP3-receptor has been identified. The use of bioinformatic analyses based on previously known sequences of IP3-receptor failed to identify potential IP3-receptor candidates in any Apicomplexa. In this work, we combine the biochemical approach of an IP3 affinity chromatography column with bioinformatic meta-analyses to identify potential vital membrane proteins that present binding with IP3 in Plasmodium falciparum. Our analyses reveal that PF3D7_0523000, a gene that codes a transport protein associated with multidrug resistance as a potential target for IP3. This work provides a new insight for probing potential candidates for IP3-receptor in Apicomplexa.
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