期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 13, 页码 -出版社
MDPI
DOI: 10.3390/ijms241311102
关键词
porcine; pig; spinal cord injury; contusion; compression; myelotomy; biomaterial; microfiber; conducting polymer; regeneration
Biomaterials have the potential to enhance neural repair after spinal cord injury, and testing in large animals is crucial for clinical translation. A porcine SCI model was developed to investigate the effects of myelotomy and implantation of MF/fibrin gel scaffolds. While myelotomy and lesion debridement did not further damage neural tissue, they had limited positive effects on neural regrowth. The MF/fibrin gel implant facilitated axonal sprouting and elongation, but also increased lesion volume and did not prevent fibrosis, thus limiting functional neural regeneration.
Biomaterials may enhance neural repair after spinal cord injury (SCI) and testing their functionality in large animals is essential to achieve successful clinical translation. This work developed a porcine contusion/compression SCI model to investigate the consequences of myelotomy and implantation of fibrin gel containing biofunctionalized carbon microfibers (MFs). Fourteen pigs were distributed in SCI, SCI/myelotomy, and SCI/myelotomy/implant groups. An automated device was used for SCI. A dorsal myelotomy was performed on the lesion site at 1 day post-injury for removing cloths and devitalized tissue. Bundles of MFs coated with a conducting polymer and cell adhesion molecules were embedded in fibrin gel and used to bridge the spinal cord cavity. Reproducible lesions of about 1 cm in length were obtained. Myelotomy and lesion debridement caused no further neural damage compared to SCI alone but had little positive effect on neural regrowth. The MFs/fibrin gel implant facilitated axonal sprouting, elongation, and alignment within the lesion. However, the implant also increased lesion volume and was ineffective in preventing fibrosis, thus precluding functional neural regeneration. Our results indicate that myelotomy and lesion debridement can be advantageously used for implanting MF-based scaffolds. However, the implants need refinement and pharmaceuticals will be necessary to limit scarring.
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