A Modified H-1-NMR Quantification Method of Ephedrine Alkaloids in Ephedrae Herba Samples

A previous H-1-NMR method allowed the quantification of ephedrine alkaloids; however, there were some disadvantages. The cyclized derivatives resulted from the impurities of diethyl ether were identified and benzene was selected as the better extraction solvent. The locations of ephedrine alkaloids were confirmed with 2D NMR. Therefore, a specific H-1-NMR method has been modified for the quantification of ephedrine alkaloids. Accordingly, twenty Ephedrae Herba samples could be classified into three classes: (I) E. sinica-like species; (II) E. intermedia-like species; (III) others (lower alkaloid contents). The results indicated that ephedrine and pseudoephedrine are the major alkaloids in Ephedra plants, but the concentrations vary greatly determined by the plant species and the collection locations.

Automated summary

A specific H-1-NMR method has been modified to quantify ephedrine alkaloids. Benzene was identified as the better extraction solvent to eliminate impurities. The locations of ephedrine alkaloids were confirmed using 2D NMR, and the concentrations of ephedrine and pseudoephedrine varied greatly among different species and collection locations of Ephedra plants.