期刊
VIRULENCE
卷 8, 期 1, 页码 30-40出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/21505594.2016.1199316
关键词
biofilm; Burkholderia pseudomallei; Galleria mellonella; thermal stress; trehalase; trehalose; virulence
资金
- Defense Science and Technology Laboratory [DSTLX-1000060221 (WP2)]
- Transformational Medical Technologies program contract from the Department of Defense Chemical and Biological Defense program through the Defense Threat Reduction Agency (DTRA) [W911NF-08-C-0023]
- Thailand research fund (TRF)
- Commission of Higher Education (CRE)
- Mahidol University
- TRF [MRG5480076]
- ICTM grant from The Faculty of Tropical Medicine, Mahidol University, Thailand
Trehalose is a disaccharide formed from two glucose molecules. This sugar molecule can be isolated from a range of organisms including bacteria, fungi, plants and invertebrates. Trehalose has a variety of functions including a role as an energy storage molecule, a structural component of glycolipids and plays a role in the virulence of some microorganisms. There are many metabolic pathways that control the biosynthesis and degradation of trehalose in different organisms. The enzyme trehalase forms part of a pathway that converts trehalose into glucose. In this study we set out to investigate whether trehalase plays a role in both stress adaptation and virulence of Burkholderia pseudomallei. We show that a trehalase deletion mutant (treA) had increased tolerance to thermal stress and produced less biofilm than the wild type B. pseudomallei K96243 strain. We also show that the treA mutant has reduced ability to survive in macrophages and that it is attenuated in both Galleria mellonella (wax moth larvae) and a mouse infection model. This is the first report that trehalase is important for bacterial virulence.
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