4.6 Article

Deuterated squalene and sterols from modified Saccharomyces cerevisiae

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ORGANIC & BIOMOLECULAR CHEMISTRY
卷 21, 期 32, 页码 6537-6548

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ROYAL SOC CHEMISTRY
DOI: 10.1039/d3ob00754e

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Uniformly deuterated sterols and related materials are crucial for various studies such as neutron, NMR, tracing, and bioanalysis, as well as for improving lipid nanoparticle formulations. This research focuses on producing sufficient amounts of these materials, including uniformly deuterated squalene and cholesterol with controlled deuteration levels. Additionally, new deuterated sterols (22,23-dihydrobrassicasterol and 24-methylenecholesterol) were prepared, increasing the number of characterized sterols with uniform deuteration. The report also introduces a protocol for separating sterols with different degrees of unsaturation using silylation/silver ion chromatography.
Uniformly deuterated sterols and biosynthetically related materials are important for neutron, NMR, tracing and bioanalysis studies as well as critical tools for the creation of improved lipid nanoparticle formulations. The production of sufficient quantities of materials relies not only on the engineering of microorganisms to selectively accumulate desired materials but also methods for the isolation, purification and characterisation of these materials to ensure their usefulness. Uniformly deuterated squalene, the universal precursor to sterols in biological systems, has been produced and characterised. Cholesterol has been produced with controlled levels of uniform deuteration, increased biosynthetic yield and a methodology developed for the extraction and purification of this material without HPLC. Two sterols, not previously produced in deuterated forms, have been prepared with uniform deuteration: 22,23-dihydrobrassicasterol and 24-methylenecholesterol. This report triples the number of sterols that have been produced with uniform deuteration, purified and characterised and provides a silylation/silver ion chromatography protocol for the separation of sterols which differ by the degree of unsaturation. The techniques for the C-13 NMR analysis of deuterated sterols, site-specific deuteration levels and an analysis of key biosynthetic steps based on these data are reported.

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