Characterization of boar semen microbiome and association with sperm quality parameters

Elevated levels of bacteria within fresh extended boar semen are associated with decreased sperm longevity, therefore reducing the fertility of a semen dose. The objective of this study was to characterize the bacterial communities using 16S rRNA sequencing in freshly extended boar semen samples and relate the prevalence and diversity of the microbial population to sperm quality parameters 1) between studs, 2) between pooled and single-sire doses, and 3) over a 5-day period. Eight single-sire (n = 4 per stud) and eight pooled (n = 4 per stud) non-frozen extended semen doses were obtained from two boar studs (A and B). Pooled doses were the composite of the boar's ejaculates used in single-sire doses. Doses were subsampled for 5 d post-collection. Ten negative controls of each pooled dose (n = 2) and single-sire dose (n = 8) remained sealed until the last day. Microbiome analysis was achieved by examining the V4 hypervariable region of the 16S rRNA gene of flash-frozen samples. Two evaluators determined the average sperm motility and agglutination (0: no adhesion to 3: >50% adhesion) by averaging their estimates together at 10 random locations per slide. Stud A had greater sperm agglutination (1.6 vs. 1.0 & PLUSMN; 0.1; P < 0.01) than stud B. Sperm motility decreased over the 5-day period (P < 0.01) and tended (P = 0.09) to be greater in stud B than A (67.4% vs. 61.5% & PLUSMN; 0.02%). Compared with stud A, stud B had a greater relative abundance of Proteobacteria (60.0% vs. 47.2% & PLUSMN; 1.5%; P < 0.01) and a lower relative abundance of Firmicutes (22.5% vs. 31.9% & PLUSMN; 1.4%; P < 0.01). Moreover, stud A had a greater relative abundance of Bacteroidetes (6.3% vs. 5.3% & PLUSMN; 0.4%; P < 0.01) and Actinobacteria (11.5% vs. 10.1% & PLUSMN; 0.5%; P = 0.05) than stud B. Differences were found in alpha diversity for both Chao1 (P < 0.01) and Shannon (P < 0.01) diversity indexes among days 2, 3, 4, and 5 post-collection to day 1. For beta diversity, unweighted UniFrac metric on days 2, 3, 4, and 5 post-collection differed from those on day 1 (P < 0.01). There were significant correlations between sperm motility and relative abundance of Prevotella (r = -0.29), Ruminococcus (r = -0.24), and Bacteroides (r = -0.32). Additionally, there were significant correlations between sperm motility and Chao1 (r = -0.50) and Shannon's index (r = -0.36). These results demonstrate that differences in bacterial communities over time and between boar studs can be associated with variation in sperm quality. Sperm quality measurements and the bacterial communities of 16 fresh extended boar semen doses revealed a relationship between the semen microbiome and sperm cell function which can influence semen quality. Differences in the presence of specific bacterial communities and semen microbiome diversity status were distinct between boar stud operations and over time, potentially acting as an indicator of semen dose fertility. Lay Summary The ability to improve production output remains essential to meet the growing global demand for pork. However, the presence of pathogenic bacteria such as Escherichia coli and Clostridium perfringens can hinder production goals by reducing semen quality through increased clumping events and decreased sperm motility. In addition, reduced conception rates and decreased litter size can occur when bacterial-contaminated semen doses are used for artificial insemination. The purpose of this study was to determine the bacterial communities within freshly extended boar semen and associate specific bacterial communities with sperm quality measurements. Current findings suggest that certain bacteria can accumulate within a group of animals or over a short period of time which can impact sperm cell characteristics. Having less diverse bacterial communities also appears to be associated with favorable semen quality. Future research is needed to determine the interactions different bacterial communities have with sperm cells to characterize their nature as pathogenic or commensal.

Automated summary

This study characterized the bacterial communities in fresh extended boar semen samples using 16S rRNA sequencing and associated the prevalence and diversity of the microbial population with sperm quality parameters. Differences in bacterial communities were found between boar studs and over time, and these differences were associated with variations in sperm quality. The findings suggest that the semen microbiome plays a role in sperm cell function and can influence semen quality.