期刊
SCIENTIFIC REPORTS
卷 6, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep24401
关键词
-
资金
- Agriculture Science Technology Achievement Transformation Fund [2014GB2A300003]
- Graduate Innovation Project of Shanxi Province [20143054]
- Key Scientific and Technological Grant from Shanxi Province [20130311026-6]
The co-infection of porcine reproductive respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) is quite common in clinical settings and no effective treatment to the co-infection is available. In this study, we established the porcine alveolar macrophages (PAM) cells model co-infected with PRRSV/PCV2 with modification in vitro, and investigated the antiviral activity of Matrine on this cell model and further evaluated the effect of Matrine on virus-induced TLR3,4/NF-kappa B/TNF-alpha pathway. The results demonstrated PAM cells inoculated with PRRSV followed by PCV2 2 h later enhanced PRRSV and PCV2 replications. Matrine treatment suppressed both PRRSV and PCV2 infection at 12 h post infection. Furthermore, PRRSV/PCV2 co-infection induced I kappa B alpha degradation and phosphorylation as well as the translocation of NF-kappa B from the cytoplasm to the nucleus indicating that PRRSV/PCV2 co-infection induced NF-kappa B activation. Matrine treatment significantly down-regulated the expression of TLR3, TLR4 and TNF-alpha although it, to some extent, suppressed p-I kappa B alpha expression, suggesting that TLR3,4/NF-kappa B/TNF-alpha pathway play an important role of Matrine in combating PRRSV/PCV2 co-infection. It is concluded that Matrine possesses activity against PRRSV/PCV2 co-infection in vitro and suppression of the TLR3,4/NF-kappa B/TNF-alpha pathway as an important underlying molecular mechanism. These findings warrant Matrine to be further explored for its antiviral activity in clinical settings.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据