TIPE2 regulates periodontal inflammation by inhibiting NF-kappa B p65 phosphorylation

The roles and molecular mechanisms of tumor necrosis factor-a-induced protein 8-like 2 (TIPE2) in periodontitis remain largely unknown. Objective: This study aimed to determine the expression of TIPE2 and NF-.B p65 in rat Porphyromonas gingivalis-induced periodontics in vivo. Methodology: Periodontal inflammation and alveolar bone resorption were analyzed using western blotting, micro- computed tomography, TRAP staining, immunohistochemistry, and immunofluorescence. THP-1 monocytes were stimulated using 1 mu g/ml Pg. lipopolysaccharide (Pg.LPS) to determine the expression of TIPE2 in vitro. TIPE2 mRNA was suppressed by siRNA transfection, and the transfection efficiency was proven using western blotting and real-time PCR. The NF-kappa B pathway was activated by treating the cells with 1 mu g/ml Pg.LPS to explore related mechanisms. Results: The expression of both TIPE2 and NF-.B p65 was increased in the gingival tissues of rat periodontitis compared with normal tissues. Positive expression of TIPE2 was distributed in inflammatory infiltrating cells and osteoclasts in the marginal lacunae of the alveolar bone. However, strong positive expression of TIPE2 in THP-1 was downregulated after Pg.LPS stimulation. TIPE2 levels negatively correlated with TNF-alpha and IL-1 beta. Decreased TIPE2 in THP-1 further promoted NF-kappa B p65 phosphorylation. Mechanistically, TIPE2 knockdown upregulated NF-kappa B signaling pathway activity. Conclusions: Taken together, these findings demonstrate that TIPE2 knockdown aggravates periodontal inflammatory infiltration via NF-.B pathway. Interventions aimed at increasing TIPE2 may help in the therapeutic applications for periodontitis.

Automated summary

The roles and molecular mechanisms of TIPE2 in periodontitis were investigated. The expression of TIPE2 and NF-κB p65 was found to be increased in gingival tissues of rat periodontitis. Knockdown of TIPE2 upregulated NF-κB signaling pathway activity. Therefore, increasing TIPE2 may be a potential therapeutic approach for periodontitis.