Nutritional and functional insight into novel probiotic lycopene-soy milk by genome edited Bacillus subtilis

Nutritional and functional soy-based milk gains growing attention globally in food industry. However, its poor sensorial attributes, single flavor, and limited substance variety become critical issues in displaying balanced nutrition and multifunction for health. Herein, a novel probiotic lycopene-soy milk was developed by genome edited Bacillus subtilis harboring lycopene biosynthesis cassette with efficient lycopene production of 25.73 & PLUSMN; 1.57 mg/g DCW. Further investigation displayed desirable pH, reducing sugar, protein, total phenolic content and isoflavone for achieved milk than conventional soy milk, implying it with well-balanced nutritional quality. Notably, achieved milk exhibited stronger antioxidant capacity and higher isoflavone bioavailability for functionality. Moreover, it possessed significantly high scores for taste, appearance, and overall acceptability, suggesting its excellent sensorial attributes. To our delight, it is the first time to fortify soy-milk with probiotic and lycopene by genome edited B. subtilis to explore additive effect on improving nutritional value and functionality for food application.

Automated summary

Nutritional and functional soy-based milk is gaining attention in the global food industry. However, it faces issues such as poor taste and limited variety. In this study, a novel probiotic lycopene-soy milk was developed using genome edited Bacillus subtilis, which showed improved nutritional quality and functionality compared to traditional soy milk. The lycopene-soy milk exhibited strong antioxidant capacity, higher isoflavone bioavailability, and excellent sensorial attributes.