期刊
SCIENTIFIC REPORTS
卷 6, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep27205
关键词
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资金
- Nanjing Agricultural University, Chinese Academy of Sciences [CAS 135 program] [XTBG-T02]
- Nanjing Agricultural University, Chinese Academy of Sciences [equipment RD grant] [YZ201260]
- Yunnan Provincial Government (Baiming Haiwai Gaocengci Rencai Jihua)
Biohydrogen production has received widespread attention from researchers in industry and academic fields. Response surface methodology (RSM) was applied to evaluate the effects of several key variables in anaerobic fermentation of glucose with Clostridium butyrium, and achieved the highest production rate and yield of hydrogen. Highest H-2 yield of 2.02 mol H-2/mol-glucose was achieved from 24 h bottle fermentation of glucose at 35 degrees C, while the composition of medium was (g/L): 15.66 glucose, 6.04 yeast extract, 4 tryptone, 3 K2HPO4, 3 KH2PO4, 0.05 L-cysteine, 0.05 MgSO4 . 7H(2)O, 0.1 MnSO4 center dot H2O and 0.3 FeSO4 center dot 7H(2)O, which was very different from that for cell growth. Sugarcane bagasse and Jatropha hulls were selected as typical tropical biomass wastes to produce sugars via a two-step acid hydrolysis for hydrogen production. Under the optimized fermentation conditions, H-2 yield (mol H-2/mol-total reducing sugar) was 2.15 for glucose, 2.06 for bagasse hydrolysate and 1.95 for Jatropha hull hydrolysate in a 3L fermenter for 24 h at 35 degrees C, with H-2 purity of 49.7-64.34%. The results provide useful information and basic data for practical use of tropical plant wastes to produce hydrogen.
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