Heavy water induces bundling in entangled actin networks

Heavy water is known to affect many different biological systems, with the most striking effects observed at the cellular level. Many dynamic processes, such as migration or invasion, but also central processes of cell proliferation are measurably inhibited by the presence of deuterium oxide (D2O). Furthermore, individual cell deformabilities are significantly decreased upon D2O treatment. In order to understand the origin of these effects, we studied entangled filamentous actin networks, a commonly used model system for the cytoskeleton, which is considered a central functional element for dynamic cellular processes. Using bulk shear rheology to extract rheological signatures of reconstituted actin networks at varying concentrations of D2O, we found a non-monotonic behavior, which is explainable by a drastic change in the actin network architecture. Applying light scattering and fluorescence microscopy, we were able to demonstrate that the presence of deuterium oxide induces bundling in reconstituted entangled networks of filamentous actin. This constitutes an entirely novel and previously undescribed actin bundling mechanism.

Automated summary

Heavy water, or deuterium oxide (D2O), has significant effects on various biological systems, particularly at the cellular level. It inhibits dynamic processes such as migration and invasion, as well as central processes of cell proliferation. D2O treatment also reduces individual cell deformabilities. Researchers discovered that D2O induces bundling in reconstituted entangled networks of filamentous actin, a novel and previously undescribed actin bundling mechanism.