期刊
VIROLOGICA SINICA
卷 38, 期 4, 页码 508-519出版社
KEAI PUBLISHING LTD
DOI: 10.1016/j.virs.2023.05.004
关键词
SARS-CoV-2; Interactome; Transcriptome; Integration analysis; ER stress
类别
Through manually curating published studies, we established a comprehensive network of 3,591 human proteins interacting with 31 SARS-CoV-2 viral proteins, and identified four key host factors involved in SARS-CoV-2 infection. Among them, SERPINE1 was found to facilitate SARS-CoV-2 replication and alleviate endoplasmic reticulum stress induced by ORF8 protein through interaction. These findings highlight the importance of systematic integration analysis in understanding SARS-CoV-2-human interactions.
The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has seriously threatened global public health and caused huge economic losses. Omics studies of SARS-CoV-2 can help understand the interaction between the virus and host, thereby providing a new perspective in guiding the intervention and treatment of the SARS-CoV-2 infection. Since large amount of SARS-CoV-2 omics data have been accumulated in public databases, this study aimed to identify key host factors involved in SARS-CoV-2 infection through systematic integration of transcriptome and interactome data. By manually curating published studies, we obtained a comprehensive SARS-CoV-2-human protein-protein interactions (PPIs) network, comprising 3591 human proteins interacting with 31 SARS-CoV-2 viral proteins. Using the RobustRankAg-gregation method, we identified 123 multiple cell line common genes (CLCGs), of which 115 up-regulated CLCGs showed host enhanced innate immunity and chemotactic response signatures. Combined with network analysis, co-expression and functional enrichment analysis, we discovered four key host factors involved in SARS-CoV-2 infection: IFITM1, SERPINE1, DDX60, and TNFAIP2. Furthermore, SERPINE1 was found to facilitate SARS-CoV-2 replication, and can alleviate the endoplasmic reticulum (ER) stress induced by ORF8 protein through interaction with ORF8. Our findings highlight the importance of systematic integration analysis in understanding SARS-CoV-2-human interactions and provide valuable insights for future research on potential therapeutic targets against SARS-CoV-2 infection.
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