期刊
SCIENTIFIC REPORTS
卷 6, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep22691
关键词
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资金
- National Institutes of Health [EB012597, AR057837, DE021468, HL099073, R56AI105024]
- Office of Naval Research Young National Investigator Award
- Presidential Early Career Award for Scientists and Engineers (PECASE)
- Consejo Nacional de Ciencia y Tecnologia of Mexico (CONACyT) [262130, 234713]
- Tecnologico de Monterrey, MIT International Science and Technology Initiatives (MISTI)
- Tecnologico de Monterrey, the MIT Media Lab
- NIH Transformative Award [NIH 1R01MH103910, NIH 1U01MH106011]
- New York Stem Cell Foundation-Robertson Award
- Fundacion Mexico en Harvard
- Simons Foundation Postdoctoral Fellowship
- NIH Director's Pioneer Award [1DP1NS087724]
To date, much effort has been expended on making high-performance microscopes through better instrumentation. Recently, it was discovered that physical magnification of specimens was possible, through a technique called expansion microscopy (ExM), raising the question of whether physical magnification, coupled to inexpensive optics, could together match the performance of high-end optical equipment, at a tiny fraction of the price. Here we show that such hybrid microscopy methods-combining physical and optical magnifications-can indeed achieve high performance at low cost. By physically magnifying objects, then imaging them on cheap miniature fluorescence microscopes (mini-microscopes), it is possible to image at a resolution comparable to that previously attainable only with benchtop microscopes that present costs orders of magnitude higher. We believe that this unprecedented hybrid technology that combines expansion microscopy, based on physical magnification, and mini-microscopy, relying on conventional optics-a process we refer to as Expansion Mini-Microscopy (ExMM)-is a highly promising alternative method for performing cost-effective, high-resolution imaging of biological samples. With further advancement of the technology, we believe that ExMM will find widespread applications for high-resolution imaging particularly in research and healthcare scenarios in undeveloped countries or remote places.
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