Molecular characterization of schistosome cercariae and their Bulinus snail hosts from Niakhar, a seasonal transmission focus in central Senegal

Bulinus senegalensis and Bulinus umbilicatus, two sympatric freshwater snails found in temporal ponds in Senegal, were thought to be involved in the transmission of Schistosoma haematobium and/or Schistosoma curassoni. To better understand the role of these Bulinus species in the transmission of human and animal Schistosoma species, B. senegalensis and B. umbilicatus were collected in 2015, during a malacological survey, from a temporal pond in Niakhar, central Senegal. Snails were induced to shed cercariae on two consecutive days. Individual cercariae from each snail were collected and preserved for molecular identification. Infected snails were identified by analysis of a partial region of the cytochrome c oxidase subunit 1 (cox1) gene. Six individual cercariae shed from each infected snail were identified by analyses of the cox1, nuclear ITS and partial 18S rDNA regions. Of the 98 snails collected, one B. senegalensis had a mixed infection shedding S. haematobium, S. bovis and S. haematobium- S. bovis hybrid cercariae and one B. umbilicatus was found to be shedding only S. haematobium. These data provide molecular confirmation for B. senegalensis transmitting S. bovis and S. haematobium-S. bovis hybrids in Senegal. The multiple Bulinus species involved in the human urogenital schistosomiasis in Senegal provides a high force of transmission warranting detailed mapping, surveillance and regular treatment of at-risk populations.

Automated summary

Bulinus senegalensis and Bulinus umbilicatus, two sympatric freshwater snails found in temporal ponds in Senegal, were collected and analyzed to determine their role in transmitting Schistosoma species. The results showed that B. senegalensis transmitted S. bovis and S. haematobium-S. bovis hybrids, while B. umbilicatus only transmitted S. haematobium. These findings highlight the importance of mapping, surveillance, and treatment of at-risk populations affected by multiple Bulinus species in Senegal.