A rapid abiotic/biotic hybrid sandwich detection for trace pork adulteration in halal meat extract

In this study, we prepared molecularly imprinted polymer nanogels with good affinity for the Fc domain of immunoglobulin G (IgG) using 4-(2-methacrylamidoethylaminomethyl) phenylboronic acid as a modifiable functional monomer for post-imprinting in-cavity modification of a fluorescent dye (F-Fc-MIP-NGs). A novel nanogel-based biotic/abiotic hybrid sandwich detection system for porcine serum albumin (PSA) was developed using F-Fc-MIP-NGs as an alternative to a secondary antibody for fluorescence detection and another molecularly imprinted polymer nanogel capable of recognizing PSA (PSA-MIP-NGs) as a capturing artificial antibody, along with a natural antibody toward PSA (Anti-PSA) that was used as a primary antibody. After incubation of PSA and Anti-PSA with F-Fc-MIP-NGs, the PSA/Anti-PSA/F-Fc-MIP-NGs complex was captured by immobilized PSA-MIP-NGs for fluorescence measurements. The analysis time was less than 30 min for detecting pork adulteration of 0.01 wt% in halal beef and lamb meats. The detection limit was comparable to that of frequently used immunoassays. Therefore, we believe that this method is a promising, sensitive, and rapid detection method for impurities in real samples and could be a simple, inexpensive, and rapid alternative to conventional methods that have cumbersome procedures of 4 hours or more.

Automated summary

In this study, molecularly imprinted polymer nanogels with good affinity for immunoglobulin G (IgG) were prepared by modifying a functional monomer with a fluorescent dye. A sandwich detection system for porcine serum albumin (PSA) was developed using these nanogels as a secondary antibody, along with another nanogel capable of recognizing PSA and a natural antibody as the primary antibody. The method showed promising results in detecting pork adulteration in halal beef and lamb meats, with a detection limit comparable to traditional immunoassays.