Sustainable bioactivity enhancement of ZnO-Ag nanoparticles in antimicrobial, antibiofilm, lung cancer, and photocatalytic applications

Cancer, microbial infections, and water pollution are significant challenges the modern human population faces. Traditional treatments for cancer and infections often have adverse effects and ecological consequences, while chemical methods for water decontamination can produce harmful byproducts. Metal nanoparticles, particularly zinc oxide (ZnO) and silver (Ag) nanoparticles, show promise in addressing these issues. However, doping Ag on ZnO NPs may synergistically enhance biomedical and therapeutic effects with fewer adverse consequences and improved photocatalytic properties for wastewater treatment. This study aimed to create ZnO and ZnO-Ag nanoparticles through green synthesis and compare their anticancer, antimicrobial, and photocatalytic activity mechanisms. XRD studies determined the crystal diameters of ZnO NPs and ZnO-Ag NPs to be 12.8 nm and 15.7 nm, respectively, with a hexagonal wurtzite structure. The XPS and EDS analyses confirmed the presence of Ag on the ZnO NPs. ZnO NPs and ZnO-Ag NPs exhibited low aggregation in aqueous suspensions, with zeta potentials of -20.5 mV and -22.7 mV, respectively. Evaluating antimicrobial and antibiofilm activity demonstrates that ZnO-Ag NPs have superior potential to ZnO NPs and standard antibiotic drugs against E. coli, S. typhi, B. subtilis, S. aureus, C. albicans, and A. niger. The results of the in vitro cytotoxicity test indicated that on the NCI-H460 lung cancer cell line, ZnO NPs and ZnO-Ag NPs demonstrated IC50 values of 40 & mu;g mL-1 and 30 & mu;g mL-1, respectively. The photocatalytic degradation of methylene blue under direct sunlight revealed that ZnO and ZnO-Ag NPs degraded MB by 98% and 70% in 105 min, respectively. These results show that these nanomaterials may have great potential for treating the aforementioned issues. Green synthesized ZnO and ZnO-Ag NPs using a plant extract. SEM image validate the final product. Characterized by FTIR, XRD, zeta, XPS, etc. Following this, their anticancer, antibacterial, and photocatalytic potentials are evaluated.

Automated summary

This study synthesized ZnO and ZnO-Ag nanoparticles using a green method and compared their anticancer, antimicrobial, and photocatalytic activity mechanisms. The results showed that ZnO-Ag nanoparticles exhibited superior potential to ZnO nanoparticles and standard antibiotic drugs in combating various microbial strains. These nanomaterials have great potential for treating cancer, microbial infections, and water pollution.