期刊
FOOD HYDROCOLLOIDS
卷 145, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodhyd.2023.109101
关键词
Nanostructured lipid carriers; Candelilla wax; Pea protein isolate; D -limonene; Antifungal activity; High stability
The objective of this work was to develop nanostructured lipid carriers (NLCs) with preservation effect using pea protein isolate (PPI) nanoparticles as emulsifiers, candelilla wax (CW) as organo-gelator, and D-limonene (DL) as an antifungal agent. The optimal formulation parameters of NLCs were determined based on particle size, poly-dispersity index, and zeta potential, and further characterization of NLCs was performed. The addition of CW in NLCs resulted in improved dispersion, storage stability, and inhibition of DL release.
The objective of this work was to develop nanostructured lipid carriers (NLCs) with preservation effect by using pea protein isolate (PPI) nanoparticles as emulsifiers, candelilla wax (CW) as organo-gelator and D-limonene (DL) as antifungal agent. The optimal formulation parameters of NLCs were firstly determined based on results of particle size, poly-dispersity index and zeta potential, and then NLCs prepared by optimal formulation were further characterized. The results showed that DL was successfully encapsulated inside NLCs. Moreover, the encapsulation of amorphous DL led to more disordered crystalline state of NLCs. In addition, in comparing the properties of NLCs and nanoemulsions (NEs) without CW, it was found that the addition of CW seemed to have several beneficial effects on NLCs: 1) NLC droplets were well dispersed, whereas NE droplets exhibited coalescence and flocculation; 2) compared with NEs, NLCs displayed excellent storage stability and inhibition ability of DL release. Furthermore, antifungal activity of NLCs was observed by in vitro and in vivo study. The acquired information in current research can provide novel insight into development of stable and antifungal nanocarriers that own a promising application in tomato preservation.
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