SERS-active substrates using DVD-R coated in silver thin films: A preliminary study for detection of commercial glyphosate

Glyphosate (GLP) is the herbicide with the highest level of global commercialization and historical use. Even though numerous studies have found this substance to be harmless, current research demonstrates that GLP might affect human health. For this reason, researcher efforts are concentrating on alternatives for analytical quantification, such as Surface Enhanced Raman Spectroscopy (SERS). In this work, a DVD-R@AgNPs SERS substrate was produced by the Cathodic Cage Plasma Deposition (CCPD) technique, which allowed a thin film layer deposition of silver nanoparticles (AgNPs) on the PC grating structure from Digital Video/Versatile Disc Recordable (DVD-R). Scanning Electron Microscopy with energy-dispersive X-ray spectroscopy was used to characterize the substrate and chemical changes on the surface after AgNPs deposition. The DVD-R@AgNPs substrate was used to detect standard crystal violet (CV), GLP, and RoundupTM GLP (GLP-RU) using Raman Spectroscopy. The CV was used as a control sample for SERS measurement, allowing the calculation of the substrate enhancement factor, which was in the order of -105. To evaluate the efficiency of the SERS substrate, the limit of detection was calculated and showed values of -10-10 mol/L for CV, 10-7 and 10-8 mol/L for GLP, and 10-6 mol/L for GLP-RU. Thus, the DVD-R@AgNPs SERS sensor is a low-cost substrate that analyzes traces of pesticides such as commercial GLP, demonstrating high SERS sensitivities and many applications.

Automated summary

In this study, a DVD-R@AgNPs SERS substrate was prepared using the CCPD technique for detecting herbicide GLP. Scanning Electron Microscopy and Energy-dispersive X-ray spectroscopy were used to characterize the substrate and surface chemical changes. The DVD-R@AgNPs SERS sensor is a low-cost substrate that can analyze trace amounts of pesticides, demonstrating high SERS sensitivities and various applications.