NanopoReaTA: a user-friendly tool for nanopore-seq real-time transcriptional analysis

Oxford Nanopore Technologies' (ONT) sequencing platform offers an excellent opportunity to perform real-time analysis during sequencing. This feature allows for early insights into experimental data and accelerates a potential decision-making process for further analysis, which can be particularly relevant in the clinical context. Although some tools for the real-time analysis of DNA-sequencing data already exist, there is currently no application available for differential transcriptome data analysis designed for scientists or physicians with limited bioinformatics knowledge. Here, we introduce NanopoReaTA, a user-friendly real-time analysis toolbox for RNA-sequencing data from ONT. Sequencing results from a running or finished experiment are processed through an R Shiny-based graphical user interface with an integrated Nextflow pipeline for whole transcriptome or gene-specific analyses. NanopoReaTA provides visual snapshots of a sequencing run in progress, thus enabling interactive sequencing and rapid decision making that could also be applied to clinical cases. Availability and implementation: Github https://github.com/AnWiercze/NanopoReaTA; Zenodo https://doi.org/10.5281/zenodo.8099825.

Automated summary

Oxford Nanopore Technologies' sequencing platform allows for real-time analysis, providing early insights and accelerating decision-making for further analysis. NanopoReaTA is introduced as a user-friendly toolbox for real-time analysis of RNA-sequencing data from ONT, offering visual snapshots and enabling interactive sequencing and rapid decision-making.