期刊
BIOCELL
卷 47, 期 7, 页码 1549-1560出版社
TECH SCIENCE PRESS
DOI: 10.32604/biocell.2023.027308
关键词
RASAL2; Cervical cancer; Knockdown; Silencing; Tumor suppressor
类别
This study aimed to investigate the role of RASAL2 in cervical cancer (CC). The expression of RASAL2 mRNA and protein was analyzed in CC tissues, cell lines, and normal cervical cells. The study found that down-regulation of RASAL2 was associated with advanced stage and metastasis of CC, while its over-expression inhibited proliferation and metastasis of CC cells. Silencing of RASAL2 expression increased the proliferation, invasion, and migration of CC cells.
Background: This study was designed to investigate the roles of RASAL2 in cervical cancer (CC). Methods: Fifty-four CC tissues and 33 adjacent tissues were obtained from CC patients admitted to our hospital between March 2012 and June 2014. Real-time polymerase chain reaction and western blotting were performed to analyze the expression of RASAL2 mRNA and protein in these tissues, CC cell lines, and normal cervical cells. Over-expression and silencing of RASAL2 were induced after transfection, and the migration, invasion, and proliferation of the CC cell lines were examined. Results: RASAL2 mRNA and protein expressions were significantly down-regulated in CC tissues and cell lines than in adjacent tissues and normal cervical cells, respectively. While low RASAL2 expression correlated with advanced stage and metastasis of CC, its over-expression significantly inhibited proliferation and metastasis of CC cells and induced apoptosis. Under in vitro conditions, silencing of RASAL2 expression could significantly increase the proliferation, invasion, and migration of CC cells. Conclusion: RASAL2 functioned as a tumor suppressor in CC, and was down-regulated in CC tissue samples and cell lines.
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