期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 302, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2023.123012
关键词
Thiophenols; M-beta-CD; ESIPT; Fluorescent probe
类别
In order to detect the level of thiophenols in the environment and biological samples, probes 1a-b were synthesized by introducing the 2,4-dinitrophenyl ether group into diethylcoumarin-salicylaldehyde based compounds. These probes can form host-guest compounds with methylated beta-cyclodextrin, showing significant increase in fluorescence intensity in thiophenols detection. The hydrophobic cavity of methylated beta-cyclodextrin enhances the fluorescence intensity of probes 1a-b and reduces their detection limits to thiophenols, without affecting their selectivity and response time.
Thiophenol and its derivatives are compounds with high toxicity to organisms and environmental pollution, so it is necessary to detect the level of thiophenols in the environment and biological samples. The probes 1a-b were obtained by introducing the 2,4-dinitrophenyl ether group into diethylcoumarin-salicylaldehyde based compounds. And they can form host-guest compounds with methylated beta-cyclodextrin (M-beta-CD), the association constants of inclusion complexes are 49.2 M-1, 125 M-1 respectively. The fluorescence intensities of probes 1a-b at 600 nm (1a) and 670 nm (1b) increased significantly in thiophenols detection. Meanwhile, with the addition of M-beta-CD, the hydrophobic cavity of M-beta-CD significantly increased the fluorescence intensity of probes 1a-b, thus the detection limits of probes 1a-b to thiophenols were reduced from 410 nM, 365 nM to 62 nM, 33 nM respectively. Whereas, the good selectivity and short response time of probes 1a-b towards thiophenols was not affected in the presence of M-beta-CD. Moreover, probes 1a-b were used for further water sample detection and HeLa cell imaging experiments due to their good response to thiophenols and the results suggested that probes 1a-b had the potential to detect the content of thiophenols in water samples and living cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据