In an attempt to better elucidate the material-cytoskeleton crosstalk during the initial stage of cell adhesion, here we report how suspended cells anchored to point-like bonds are able to assemble their cytoskeleton when subjected to mechanical stress. The combination of holographic optical tweezers and digital holography gives the cell footholds for adhesion and mechanical stimulation, and at the same time, acts as a label-free, force-revealing system over time, detecting the cell nanomechanical response in the pN range. To confirm the formation of the cytoskeleton structures after the stimulation, a fluorescence imaging system was added as a control. The strategy here proposed portends broad applicability to investigate the correlation between the forces applied to cells and their cytoskeleton assembly process in this or other complex configurations with multiple anchor points.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据