A mechanical biosensor based on membrane-mediated magneto-stress-electric coupled sensitization for human serum albumin detection

Recently, mechanical biosensors have attracted more attention on single molecule detection due to its high accuracy, low cost, and convenience. However, the sensitivity of the mechanical biosensors restricted their clinical application. Herein, a mechanical biosensor based on membrane-mediated magneto-stress-electric coupled sensitization (MSEC-MMB) was developed to enhance performance. Through introducing Fe3O4 nanoparticles (MNPs) to traditional stress-electric biosensors and applying a magnetic field, a magneto-stress-electric coupled biosensing system was constructed. The sensitivity of the MSEC-MMB was improved via enhancing the deformation of the mechanical membrane, which was demonstrated by detecting HSA. The optimal limit of detection (LOD) was 24 pg mL-1 under a magnetic field of 50 mT. The LOD was significantly 1 order of magnitude lower than that without the magnetic field. Besides, the MSEC-MMB showed a high specificity, selectivity, and stability. The clinical proteinuria samples were accurately detected, suggesting a good practicability of the MSEC-MMB. All these results proved the high sensitivity and practicality of the MSEC-MMB and provide a platform for early nephropathy diagnosis. The membrane-mediated magneto-stress-electric-coupled-sensitization biosensor (MSEC-MMB) was developed with a LOD of 24 pg mL-1 for HSA. The biosensor showed a high sensitivity and practicality, providing a platform for early nephropathy diagnosis.

Automated summary

Recently, a mechanical biosensor based on membrane-mediated magneto-stress-electric coupled sensitization (MSEC-MMB) was developed to improve the sensitivity and practicality of single molecule detection in clinical applications. The MSEC-MMB showed enhanced sensitivity by introducing Fe3O4 nanoparticles and a magnetic field to the traditional stress-electric biosensors, resulting in a LOD of 24 pg mL-1 for HSA. The MSEC-MMB also demonstrated high specificity, selectivity, and stability, making it a promising platform for early nephropathy diagnosis.