期刊
RSC ADVANCES
卷 6, 期 88, 页码 85074-85082出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6ra17510d
关键词
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资金
- National Natural Science Foundation of China [41301332]
- Key Laboratory of Reservoir Aquatic Environment, Chinese Academy of Science [RAE2014CB05B]
- Outstanding Talent Plan of Fudan University [JJH1829029]
- China Postdoctoral Science Foundation [2015M571600]
This study newly isolated a bacterial stain of Bacillus flexus S1 which had abilities of synthesizing lipopeptides without any inductive operations, and thus it could effectively save operational procedures or cost. This microorganism produced 1.53 g L-1 of lipopeptides with a basal medium, which was 2.59-fold higher than that with an inductive medium. Meanwhile, the sfp gene was highly expressed under non-inductive conditions. The results firstly noted micellization characteristics of a biosurfactant, including a 46.04 mg L-1 critical micelle concentration, the main chemical structure, and micelles mainly distributing at 30.2-52.5 nm and 181.9-239.9 nm. The lipopeptides indicated good stability over a temperature range of 30-50 degrees C and pH range of 6-9. Furthermore, monatomic (K+ and Na+) and divalent (Ca2+ and Mg2+) ions respectively alleviated generating smaller and bigger micellar aggregates. The lipopeptides could effectively increase the solubility of hydrophobic organic carbons. As the lipopeptides were adopted together with oil-degrading bacteria, it increased the hydrophobicity of bacterial cells by 1.92-3.04 fold, and had low toxicity to bacterial cells. This study not only provided a new lipopeptides producing bacterium which did not need any conductive operations, but also systematically investigated the characteristics of lipopeptides, providing impactful significance for commercial application.
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